In Vienna, the Duke temporarily steps down, delegating absolute power to his strict deputy, Angelo, while remaining incognito as a friar to observe the outcomes. Angelo immediately enforces dormant laws against moral laxity, condemning the young gentleman Claudio to death for premarital relations with Juliet. Claudio begs his pious sister, Isabella, a novice nun, to intercede with the deputy.
When Isabella pleads for her brother, Angelo proposes a dark trade: he will spare Claudio if Isabella yields her chastity to him. Desperate and resolute, Isabella refuses, choosing her spiritual integrity over her brother's life, and resolves to let Claudio die with honor. Meanwhile, the disguised Duke devises a plan to trap Angelo by substituting Mariana, Angelo's long-abandoned and still-betrothed former partner, into the nocturnal rendezvous.
In the prison, despite the substitution, the corrupt Angelo still sends a warrant for Claudio's execution, though the provost secretly saves Claudio by executing a pirate instead. The complex deception culminates at the city gates where the Duke returns publicly. Isabella and Mariana accuse Angelo before the sovereign, and the dark web of hypocrisy and manipulation is gradually untangled.
Ultimately, the Duke reveals his disguise, exposes Angelo's crimes, and orders him to marry Mariana immediately before facing justice. Mercy is extended where genuine repentance takes place, and the relationships are restored through a balanced resolution that emphasizes personal responsibility and forgiveness over absolute punishment.